y 1 cell line  (ATCC)

Journal: Cancers

Article Title: Targeting Oncogenic Wnt/β-Catenin Signaling in Adrenocortical Carcinoma Disrupts ECM Expression and Impairs Tumor Growth

doi: 10.3390/cancers15143559

Figure Lengend Snippet: Xenograft implantation and tumor progression: ( A ). In vivo injection process ( B ). Adrenals and the growth of tumors were monitored using ultrasound, with a 3D reconstruction of the tumor boundaries when a tumor was detected ( C ). Here, tumor boundaries mapped from ultrasound prior to necropsy (left panels). Dashed yellow circles in middle panels show macroscopic appearance of the tumors before resection. Left panels: macroscopic gross appearance of the tumors after resection. ( D ). Ultrasound tumor volume and tumor volume calculated from caliper measurements are strongly correlated (linear regression R 2 = 0.877). ( E ). In vivo bioluminescent signal is plotted against ultrasound tumor volume (NCI-H295R linear regression R 2 = 0.29; Y1 linear regression R 2 = 0.0378).

Article Snippet: NCI-H295R (RRID:CVCL_0458) and Y1 cell lines were obtained from American Type Culture Collection (Manassas, VA, USA) and cultured in a humidified incubator containing 5% CO 2 at 37 °C.

Techniques: In Vivo, Injection

Journal: Cancers

Article Title: Targeting Oncogenic Wnt/β-Catenin Signaling in Adrenocortical Carcinoma Disrupts ECM Expression and Impairs Tumor Growth

doi: 10.3390/cancers15143559

Figure Lengend Snippet: Histology of primary tumors and metastatic growths: ( A ). NCI-H295R tumors were characterized as composed of an expansile and focally infiltrative proliferation of packets, clusters, and sheets of neoplastic epithelial cells interspersed with variably sized cystic spaces. ( B ). Ki67 staining of an NCI-H295R tumor invading the adjacent kidney ( C ). Representative SF-1 staining of NCI-H295R tumors. ( D ). Tumors formed from Y1 cells were composed of ribbons, clusters, and lobules of poorly differentiated epithelial cells separated by a fine fibrovascular stroma, with multifocal areas of hemorrhage and necrosis. ( E ). Y1 Ki67 staining of tumor and adjacent kidney. ( F ). Representative SF-1 staining of a Y1 tumor. ( G , H ). NCI-H295R liver metastasis, overlain with a layer of hepatocytes. ( I ). Anti-human Nucleolar staining of NCI-H295R metastasis. ( J ). SF-1 staining of NCI-H295R metastasis. ( K ). Y1 lung metastasis. ( L ). Y1 liver metastasis. ( M , N ). SF-1 staining of Y1 metastases. ( O ). NCI-H295R xenograft tumor with COL11A1 and SF-1 immunofluorescent staining demonstrates co-expression in ACC cells. Scalebar, 100 µM. Arrowheads indicate metastatic lesions in panels H and K.

Article Snippet: NCI-H295R (RRID:CVCL_0458) and Y1 cell lines were obtained from American Type Culture Collection (Manassas, VA, USA) and cultured in a humidified incubator containing 5% CO 2 at 37 °C.

Techniques: Staining, Expressing